DYKDDDDK (FLAG) tag is an epitope tag designed for fusion proteins, which usually does not affect the activity of conjugated proteins and is widely used for protein purification. FLAG tag is also widely used in various immunoassays for target protein detection.

VHH (variable domain of heavy chain of heavy chain-only antibody) is 4 nm in length, 2.5 nm in width, and have a molecular weight of 12-15 kDa, only one tenth the size of conventional antibodies. VHH exhibits high specificity and affinity and outperforms conventional antibodies in many aspects, such as high stability, great specificity, and an easy and feasible development process.

Anti-DYKDDDDK (FLAG) VHH agarose beads are agarose beads covalently coupled with VHH antibodies, which have high specificity and affinity for FLAG. These beads can efficiently capture and separate FLAG-tagged proteins, along with associated proteins, from cell extracts of mammals, plants, bacteria, yeast, insects, and other organisms.

IP, Co-IP

Beads size: 45-165 μm (4% cross-linked agarose beads)

Storage buffer: 1xPBS pH7.4, 25% glycerol, 0.02% sodium azide.

Antibody: anti-DYKDDDDK (FLAG) VHH antibody (fused with 6xhis tag)

Binding capacity: 10 μL slurry bind 15-20 μg of DYKDDDDK (FLAG) tagged protein.

Specificity: Selectively recognizes DYKDDDDK (FLAG) fusion protein.

Host: Alpaca

Class: Recombinant